For quantitative determination of acetylcholinesterase activity. -Detection range: 10 to 600 U/L AChE activity in 96-well plate assay -Can be readily automated as a high-throughput assay for thousands of samples per day Acetylcholinesterase (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into choline and acetic acid, a reaction necessary to allow a cholinergic neuron to return to its resting state after activation. Studies show serum cholinesterase activity may relate to insecticide exposure or to one of a number of variant genotypes. As a result, cholinesterase levels of cells and plasma are used as a guide in establishing safety precautions relative to exposure and contact, as well as a guide in determining the need for workers to be removed from areas of contact with the organic phosphate insecticides.Simple, direct and automation-ready procedures for measuring AChE activity are highly desirable.BioAssay Systemsâ QuantiChromâ„ Acetylcholinesterase Assay is based on an improved Ellman method in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with 5,5â-dithiobis(2-nitrobenzoic acid). The intensity of the product color, measured at 412 nm, is proportional to the enzyme activity in the sample.
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