Second-Strand cDNA Synthesis Kit-dNTP/dUTP Based

Description

* Second Strand cDNA Synthesis Kit is an efficient system of generating double stranded cDNA from first strand cDNA as a template.* The E. coli RNase H nicks the RNA in the DNA:RNA hybrid, while the E. coli DNA Polymerase replaces the RNA with deoxyribonucleotides. The E. coli DNA Ligase completes the double stranded DNA formation by linking the gaps between the newly synthesized cDNA strand.* The dNTP based kit (Cat. No. G475) and dNTP/dUTP based kit (Cat. No. G476) provides different combination of deoxyribonucleotides tailored to the end user's needs and application.* This kit is provided as individual enzymes to meet the customer's needs and to provide maximum efficiency and flexibility in the RNA sample preparation. (Noted that these enzymes may still contain E. coli gDNA) The double stranded cDNA end product can subsequently be converted to blunt ended DNA fragments using crb's DNA End Repair Kit (Cat. No. G477), followed by abm's dA Tailing Kit (Cat. No. E009) to generate DNA suitable for whole genome sequencing.