" Distinguishes between apoptotic and healthy cells -Fluorescence microscopy and Flow cytometry: FITC (Ex/Em = 488/530 Â 30 nm) -and (optional) PI (Em = 488/590 Â 42 nm) -Mammalian -Convenient sizes (25 and100 assays) -Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis -Provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis -Highly sensive and detects apoptosis in living cells Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoCaptureâ„ Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoCaptureâ„, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm)."
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