BioVision Glycogen Colorimetric/Fluorometric Assay Kit, K646-100

Description

Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)Species reactivity- Mammalian Applications- The assay can detect glycogen 0.0004 to 2 mg/ml. Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in Î-1,4 linkage, with branching via Î-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. The BioVision Kit is an easy and accurate assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (570 nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2 mg/ml.