30223-1 - NxGenâ„ T7 RNA Polymerase - NxGen T7 RNA Polymerase, Lucigen - Each

Description

" Lucigenâs NxGenâ„ T7 RNA Polymerase is superior in transcription reliability. T7 RNA Polymerase catalyzes the 5â²â’3â² RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity. Performance Specifications: 1) Unit Concentration: 50,000 U/mL. 2) Purity (SDS-PAGE): > - 99%. 3) SS Exonuclease: 500 U < - 0.1% released. 4) DS Exonuclease: 500 U < - 0.1% released. 5) Endonuclease: 500 U < - 10% converted. 6) RNAse Contamination: 500 U = none detected. 7) E. coli 16S rDNA Contamination: 500 U < - 10 copies. 8) Storage: -20°C. Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene. Ordering information: Supplied at 50,000 U/mL in 50 mM Tris-HCl, 100 mM NaCl, 1 mM dithiothreitol, 1 mM EDTA, 50% glycerol, 0.1% Triton X-100, pH 7.9 @ 25°C. Also provided is 10X T7 RNA Polymerase Buffer which in 1X form is composed of 40 mM Tris-HCl, 6 mM MgCl2, 10 mM Dithiothreitol, 2 mM Spermidine, pH 7.9 @ 25°C. 125,000 U is supplied as 5 × 25,000 U. Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of ATP into acid-precipitable material in 1 hour at 37°C."