Growth Hormone, Recombinant, Rat (rGH) (Recombinant Protein)

Description

*Purity: Highly Purified 97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.; *Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of Rat Growth Hormone as a Reference Standard.; *Reconstitution: It is recommended to reconstitute the lyophilized Rat-GH in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.; *Seq: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile. ; *Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. ; *Accession#: NP_112300.2; NM_031038.3; P30969; *Description: A polypeptide that is secreted by the adenohypophysis. Growth hormone, also known as somatotropin, stimulates mitosis, cell differentiation and cell growth. Species-specific growth hormones have been synthesized. GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF. GH also enhances production of TNF-alpha, generation of superoxide anions from peritoneal macrophages, and natural killer activity. Recombinant Rat GH is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 21940.01 Dalton. ; *NCBI Summary: induces the synthesis of pituitary gonadotropins; G-protein coupled receptor activity acts via both G(q) and G(s) signaling pathways [RGD, Feb 2006]; *Uniprot Summary: Function: Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormo...